ANALISIS UJI MPN BAKTERI Escherichia coli PADA SAMPEL PANGAN JAJANAN ANAK SEKOLAH (PJAS) DI KOTA PONTIANAK

Abstract

Street food for children's school, known as PJAS (Pangan Jajanan Anak Sekolah), is one of the dietary staples among the Indonesian populace, particularly among children and adolescents. These food items are highly susceptible to contamination by microorganisms, posing potential health hazards if consumed. Escherichia coli is one such microbe capable of contaminating food and beverages, leading to various illnesses. This research aims to (1) determine the procedure for testing Escherichia coli MPN (Most Probable Number) in PJAS samples following SNI ISO 7251:2012 standards, and (2) understand the mechanisms behind Escherichia coli contamination in street school food. The research methodology involves MPN analysis of Escherichia coli, comprising presumptive, confirmatory, and indole reagent (Kovac’s) tests. Samples were collected from various schools in Pontianak City. The results revealed that out of the PJAS samples tested, two samples failed to meet safety standards: Es cincau (011) with an MPN >110 MPN/ml, and Es kopyor (012) with an MPN of 4.3 MPN/ml.